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Re: [ccp4bb]: zinc-finger protein crystallization
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Take a look at the crystallization of Zif268 by Pabo et al. They used
pretty drastic measures but it worked very well.
On Mon, 25 Sep 2000, xinqi liu wrote:
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> Dear all,
> I am doing a zinc-finger protein crystallization.
> When I purify it, this protein often aggregate and
> precipitate even in enough beta-mercaptoethanol (5mM),
> because of the free cysteines in protein. I use vapor
> diffusion method to do crystallization screen. But
> nearly in all drops the protein will precipitate in
> one or two days. Does anyone has good idea to make it
> become stable? Thank you very much.
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