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Re: [ccp4bb]: Anomalous data?
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> We recently collected MAD data sets at CHESS(F2) on a
> protein crystal soaked in an Os solution. The EXAFS scan before the
> data collection showed the presence of Os atoms in
> the crystal. Now when we use scalepack to find how good
> the anomalous differences are, the output says the differences
> are zero! What does this mean? Could random binding of Os
> give such a result? Thanks for any ideas/tips.
Certainly: the EXAFS scan does not distinguish where the Os atoms are -
they could be in the cryo-protectant, for all you know. Did you
back-soak?
Cheers
phx.