[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
Re: [ccp4bb]: High vs. low symmetry
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
>
> R R-free
> Start 34.9 38.4
> After TLS 27.4 31.6
> After restrained 24.7 29.1
>
> HOWEVER, when I work up my data in p1 with the same cell now having 4
> monomers in the ASU (R-merge 6.0 %) the refinement looks much better
> (monomers positioned with MOLREP):
>
> R R-free
> Start 35.8 34.6
> After TLS 29.7 28.7
> After restrained 23.4 24.7
>
That Free R is very misleading. You will obviously have pseudo 222
symmetry, and it is essential that the FreeR set retains that pseudo
symmetry. ie you need to work out how to make sure the related
reflections have the same FreeR. There are ways - easy if your P1 cell
is essentially the same as the P222 one - otherwise quite tricky..
If the cells are the same you can use CAD to extend the P222 FreeR flags
to outlim sPAC P1, then add the set using cad again to your "P1" data..
Eleanor