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Re: [ccp4bb]: Ions in the internal protein cavities - addendum 2
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On Thu, 31 May 2001, Petr Leiman wrote:
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> I did not mentioned the crystallization solution components because
> there is not way how that thing could have got into the inside of the
> protein through the surrounding hydrophobic jungle.
NEVER underestimate protein flexibility. Xenon and other heavy atom
derivatives find their way into hydrophobic cores, O2 finds its way into the
completely inaccessible binding site of hemocyanin (so do the copper ions)
etc. You mystery compound may even have been build in during trimer formation.
> The protein is an
> SDS- and urea-resistant trimer. The trimer sits on a crystallographic
> three-fold axis in space group R32.
> The crystals were obtained at pH 5.6 and at pH 8.0, with FeCl3 or CeCl
> and without them. Buffers - TrisCl and Jeffamine.
What was the precipitant? (assuming it is not Jeffamine)
Dept. of Medical Microbiology & Immunology
University of Alberta
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