[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
Re: [ccp4bb]: dehydration of crystals
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
On Fri, 1 Jun 2001 cdealwis@utk.edu wrote:
> Has anyone had experience with improved resolution after dehydrating
> crystals by transferring the crystals to high precipitant concentrations?
i and colleagues have noticed improved resolution after replacing a
fraction of the water with a cryoreagent, I assume this has the same
dehdyrating effect you may have been seeing with precipitant (after all
one person's cryo reagent is another's precipitant). i have also noticed
in a couple cases a beneficial space group change to a smaller unit
cell. by this i don't mean a few percent shrinkage, but a N-fold
reduction as noncrystallographic symmetry becomes crystallographic.
=======================================================================
"Liberation from superstition is a necessary but not
sufficient condition for science." - Carl Sagan
=======================================================================
David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
djs63@cornell.edu