Re: [ccp4bb]: dehydration of crystals

[Ravichandran Sankaranarayanan <sankaran@fas.harvard.edu>]

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Hi,

It was reported in one of the article that...'dehydration of a protein
crystal resulted in improved diffraction resolution' by Novel cryo-soaking
method. The author(s) achieved this by air-drying (??) the precipitant
(mother liquor). [Ref: Eom. et al. (1999) Acta Cryst D55 Pages 1601-1603].

Well, I raised the same query in one of the old BB message:

http://xtal.pharm.nwu.edu/~lists/ccp4list/Oct_99/0019.html

Cheers,
Ravi

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On Fri, 1 Jun 2001 cdealwis@utk.edu wrote:

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> 
> Colleagues
> Has anyone had experience with improved  resolution after dehydrating
> crystals by  transferring the crystals to high precipitant concentrations?
> Chris Dealwis (Assistant Professor)
> University of Tennessee Knoxville
> Department of Biochemistry and  Cellular Molecular Biology
> F433
> M407
> Knoxville
> TN 37996
> tel:-(865) 974 4088
> lab tel:- (865) 974 4667
> fax-(865) 974 6306
> e-mail cdealwis@utk.edu
>