Re: [ccp4bb]: twin crystal growth

[Mukesh Kumar <mukeshk@apsara.barc.ernet.in>]

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Yes, in certain proteins there is a correlation between growth rate and
twinning of the crystal. For example, in case of tetragonal lysozyme,
increasing the salt (NaCl) from say 5% to 7-8% in the reservoir (of a
hanging drop vap diffusion expt) results in large amount of twinned
crystals.  
Changing pH may also affect the twining behaviour of crystal, as is true
again with lysozyme. 
Mukesh

On Sat, 1 Apr 2000 MBTPING@ccvax.sinica.edu.tw wrote:

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> 
> Hi all.
> 
> I think I read about someone suggesting a slower grwoth rate to get the
> crystal with lower twin fraction or even completely untwinned crystal.
> So is there any correlation between the growth rate and twinning faction?
> Can I ask for some reference papers if anyone knows about them?
> 
> Ping Ko
> Research assistant, Inst of Biol Chem
> Academia Sinica, Taipei, Taiwan
>