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Re: [ccp4bb]: removeing ncs
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Leonard Thomas wrote:
>
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> Hi all,
>
> Just a simiple (I think) question about using NCS restraints. I have a
> structure that has 2 mol/asymm. I have been using NCS retraints sofar
> with pretty good results. My question is when do most people remove
> the restraints, if they are removed at all. The compound is not a dimer
> so there must be some difference in the molecules.
>
> Thank you for your comments.
> Len
>
> --
> Leonard M. Thomas Ph.D. Phone (816)235-2571
> University of Missouri - Kansas City Fax (816)235-1503
> School of Biological Sciences email thomasle@umkc.edu
> 5100 Rockhill Road, Kansas City, MO 64110
Depends on the resolution of your data - at 3A - wouldnt release at
all;
at 2.0, when the Rfactors have converged..
You should see differences in the maps then I think
Eleanor
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Eleanor J.Dodson, Chemistry Department, University of York, U.K.
Tel: Home +44 (1904) 42 44 49, work: +44 (1904) 43 25 65
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