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Re: [ccp4bb]: Deleted C terminus
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Dear Jan,
if you are sure that your missing C-terminal oxygens are not due to some
refinement
program artefact, you should consider radiation damage.
High-dose synchrotron radiation on cryo-colled crystals may lead to a
decarboxylation of carboxylic acids,
and probably as well of the C-terminus.
You will find some information in:
Burmeister, W. P.
Structural changes in cryo-cooled protein crystals owed to radiation damage
Acta Cryst D 56, 328-341 (2000).
Greetings
Wim Burmeister
*******************************************************************************
Wim Burmeister Maître de Conférence
c/o EMBL Université Joseph Fourier
B.P. 181 6, rue Jules Horowitz Faculté de Pharmacie
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