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[ccp4bb]: Trouble in interpreting self-ratation map...
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dear CCP4BB readers
Although this may not be related to CCP4, I need your advice
on the following puzzle.
I have a triclinic crystal of which structure can (hopefully...)
be solved by molecular replacement. According to matthews-coef
, the protein is possibly a hexamer(3.0) ~ octamer(2.3).
(Gel filtration specified it's a hexamer.)
The problem is that I can't imagine the NCS point group by looking
at the self-rotation map. It has strong peaks at chi=180, 146.7, 119,
and 70.9. I could find 8 peaks at chi=180. The map itself is
reachable here.
http://www.geocities.com/fakeophelia/self-rotation.gif
Can someone help me to understand the possible spatial arrangement
of this multimeric protein?
I have one more question.
According to Schroder et al. (Structure, 2000, 8(6):605), they
created 3,600 search models from the interpretation of self-ratation
map and solved the structure by MR. How can one create such large
numbers of probes?
Thank you for your help in advance.
Woocheol
--
Woo-Cheol Lee
tel. +81-3-5841-5446 fax. +81-3-5841-8023
Food Eng. Lab, Univ. Tokyo