Re: [ccp4bb]: summary of OIL OUT

[Jim Pflugrath <jwp@RigakuMSC.com>]

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Hmmm, I think the summary needs a bit reordering...then then summary can
also be used for any and all crystallization problems :)

Jim


On Mon, 3 Dec 2001, hongmin LI wrote:

> Thanks for everyone to answered my oil out question. Here is a summary:
> 
> Garavito mentioned: two-phase "coacervate" system. Also Drs. Garavito,
> Gerry McDermott, and Bart Hazes suggested to dialyze the protein against
> low ionic strength.

> Boaz Shaanan had successful case to add 250-500 NaCl.


> Jeroen Mesters suggested to grow crystals at a pH far away from protein
> pI.

> Genesh Natrajan suggested to grow crystal at pH close to protein's pI.

> 
> Edward Berry, David J. Schuller, Gerry McDermott, Mary Li, and Phoebe
> Rice pointed out to use detergent.
> 
> David J. Schuller also suggested to use MPD, 1,6-Hexanediol or SDS at
> low concentration.
> 
> Valerie Pye suggested to use metal ions.