Garavito mentioned: two-phase "coacervate" system. Also Drs. Garavito, Gerry McDermott, and Bart Hazes suggested to dialyze the protein against low ionic strength.
Jeroen Mesters suggested to grow crystals at a pH far away from protein pI.
Edward Berry, David J. Schuller, Gerry McDermott, Mary Li, and Phoebe Rice pointed out to use detergent.
David J. Schuller also suggested to use MPD, 1,6-Hexanediol or SDS at low concentration.
Boaz Shaanan had successful case to add 250-500 NaCl.
Genesh Natrajan suggested to grow crystal at pH close to protein's pI.
Valerie Pye suggested to use metal ions.
-- ------------------------------- Hongmin LI, Ph.D. Wadsworth Center New York State Department of Health Empire State Plaza, P.O. Box 509 Albany, NY 12201-0509 Tel: 518-486-9154 Fax: 518-474-7992