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Re: [ccp4bb]: Thermostability vs Structure
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Check also Methods in Enzymology, V. 334 (2001) - this is dedicated to
thermophilic enzymes and thermostability.
Salam
At 11:53 2001-12-04 +0000, you wrote:
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>*** CCP4 home page http://www.ccp4.ac.uk ***
>
>Dear all :
>
>Sorry for posting this non-CCP4 question.
>
>I am dealing with a structure of an oxidase. I have relatively decent
>data till 1.7 A, and the model is practically refined. The protein is
>very thermostable with a half life of 2 hours at 80ºC.
>
>I have compared the structure with other "normal" oxidases, and I am not
>able to find suitable differences to explain thermostability in terms of
>structure. I have analyzed presence of disulfide bridges, salt bridges,
>packing , etc ..
>
>Is/was anybody dealing with a similar problem ?. What should be the
>factors to analyze for the explanation of thermostability ?
>
>Best regards
>
>Francisco
>
>
>
>============================================
>
>Francisco J. Enguita, Ph.D.
>Protein Crystallography laboratory
>Instituto de Tecnologia Química e Biológica
>Ap. 127
>2781-901 Oeiras
>PORTUGAL
>Phone : 351-21-4469669
>Fax : 351-21-4411277
>E-mail : fenguita@itqb.unl.pt
>
>============================================
--------
Salam Al-Karadaghi, Assoc. Prof.
Molecular Biophysics,
Chemical Centre,
Lund University
Getingev. 60
PO Box 124, 221 00 Lund,
Sweden
Tel. Int+46 (0)462224512
Fax. Int+46 (0)462224692
Web: http://www.mbfys.lu.se
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