[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
Re: [ccp4bb]: Xtals come out on the skin of drop surface
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Aidong,
We once had the same problem and we managed to get clear drop without any skin
formation by lowering the temperature of the crystallisation and including some
glycerol in the crystallisation mixture (some 5-10%).
By the way, crystals grown on the surface of the drop were highly mosaic but
after optimisation (lower temperature-glycerol) the mosaicity dropped
significantly (see JMB, (1995) 246, 374-381).
Hope that helps
Tassos
Quoting Jason Yano <jkyano@bragg.bio.uci.edu>:
> *** For details on how to be removed from this list visit the ***
> *** CCP4 home page http://www.ccp4.ac.uk ***
>
> Aidong,
> I have had the same problem with the skin forming on the top of
> the drop. I don't know how to stop it, but I was successful in dealing
> with it. I used acupuncture needles to gently tease the skin off of the
> drops before I went in to get the crystals. The crystals that I
> dealt with had about 70% solvent content, so I had to be pretty
> delicate.
>
>
> Jason
>
> On Sat, 15 Dec 2001, OnLineHelpForm wrote:
>
> > Dear all,
> >
> > Sorry for this non-CCP4 related questions but I sincerely hope you
> guys can help me out. Recently I got my protein /DNA complex
> crystallized beautifully in PEG 1K or 3K. However, these crystals are
> relatively small and stop growing when they just come. In other words,
> I can not ever tell they are growing. To my surprise, I found there
> is a thick skin on the surface of drops and all crystals come sticking
> to that skin. It is said the skin forms out of denatured protein.
> So my question is how I can possibly make my protein happy in drops and
> hopefully facilitate their growing. Any suggestions are highly
> appreciated.
> >
> > Thanks in advance,
> >
> >
> > Aidong Han, hana@colorado.edu
> >
>
-------------------------------------------------------------------------
A.C.(Tassos) Papageorgiou, PhD phone: +358 2 333 8012 (office)
Senior Scientist fax: +358 2 333 8000
Turku Centre for Biotechnology E-mail: apapageo@btk.utu.fi
BioCity, Turku URL: http://www.btk.utu.fi/~apapageo
FIN-20521, Finland