[ccp4bb]: RE: (ccp4bb): Culture in large scale

[MICHAEL.FORSTNER@eu.pnu.com (MICHAEL FORSTNER)]

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Hi,

You might have an aeration problem, if you kept all other parameters constant
while scaling up. This is a common cause of insolubility and you might get
better results when trying either a bioreactor or a slightly different type of
vessel that improves aeration, if you have to use a shaker (e.g., erlenmeyer
flasks with indented walls rather than the standard ones).

I would recommend against 50 10mL cultures :)

Cheers,

  Michael

> -----Original Message-----
> From: lokanath@rocketmail.com at Internet-europe
> Sent: Monday, December 17, 2001 14:03
> To: ccp4bb@ccp4.ac.uk at Internet-Europe
> Subject: [ccp4bb]: Culture in large scale
>
>
> ***  For details on how to be removed from this list visit the  ***
> ***          CCP4 home page http://www.ccp4.ac.uk         ***
>
> Dear All,
>
> Sorry for posting this non-CCP4 related question.
>
> I try to express the foreign protein in E. coli.
>
> When I tried small scale expression (below than 10 ml),
> protein can be solubly expressed. However, protein is
> completely insoluble in large preparation. Therefore, I
> think the best way might be culture the cells in 50 bottles
> of 10 ml culture (500 ml culture). Please send me some
> better suggestions.
>
> Thanks in advance,
>
> Sincerely,
> Lokanath
>
>