Re: [ccp4bb]: Xtals come out on the skin of drop surface

[Juergen Bosch <jbosch@biochem.mpg.de>]

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Clemens Grimm wrote:

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> Dear all,
>
> I am working on a RNA/protein cocrystallisation project and I am
> experiencing the same skin/small crystal problem. As I have seen exactly
> the same behaviour with other RNA/protein cocrystals, I would suspect
> that this may be nucleic-acid specific. My observations are, that this
> skin originates from some kind of phase separation or fine precipitate.
> This PPT then sediments and forms the skin in which the crystals grow.
> If I reduce the precipitant concentration I get clear drops and no skin
> - but also no more crystals.
>
> Clemens
>

Hi Clemens,

have you tried to seed this clear drops ?

Juergen


--
Juergen Bosch                                    Tel -49 -89 8578 2648
Max Planck Institut fuer Biochemie               Fax -49 -89 8578 2641
Abt. Molekulare Strukturbiologie                 bosch@biochem.mpg.de
Am Klopferspitz 18a                              http://www.biochem.mpg.de/
D-82152 Martinsried, Germany