RE: [ccp4bb]: unusually high solvent content

["Bernhard Rupp" <br@llnl.gov>]

***  For details on how to be removed from this list visit the  ***
***          CCP4 home page http://www.ccp4.ac.uk         ***

grep "SOLVENT CON" *.pdb .....

I recall a cubic insulin around 70-80% SC

BR

> -----Original Message-----
> From: owner-ccp4bb@dl.ac.uk [mailto:owner-ccp4bb@dl.ac.uk]On Behalf Of
> JoaoMDias
> Sent: Wednesday, December 19, 2001 12:09 PM
> To: ccp4bb@dl.ac.uk
> Subject: [ccp4bb]: unusually high solvent content
> 
> 
> ***  For details on how to be removed from this list visit the  ***
> ***          CCP4 home page http://www.ccp4.ac.uk         ***
> 
> Dear All,
> 
> According to Matthews, (Mathews, B. W. (1968). J. Mol. Biol. 33,
> 491-497.), the Matthew's coefficient of protein crystals is usually
> comprehended between 1.7-3.5 A3/Da.
> 
> Are there many proteins with a much higher solvent content that the
> Matthews limits?
> 
> Do you have experience with protein crystals with high solvent contents?
> 
> Could you point me some references to published works, or reviews about
> protein crystal structures with unusual VM (above 70%)?
> 
> Thanks in advance and Merry Christmas,
> Joao Miguel
> 
> 
> 
>