Re: [ccp4bb]: MIR-test case

[Michael Sawaya <sawaya@mbi.ucla.edu>]

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Dear Hemant,

Proteinase K would be an excellent choice.  GdCl3, SmCl3,
and p-hydroxymercuribenzoic acid make high quality derivatives.
We used proteinase K and derivatives in a structural molecular
biology lab course recently given at UCLA.
Instructions for crystallization, heavy atom preparation, and where
to buy proteinase K can be found at:

http://www.doe-mbi.ucla.edu/~sawaya/m230d/Crystallization/crystallization.html

Good luck!
Mike Sawaya

Hemant Yennawar wrote:

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>
> Hello X'tallographers,
>      Before trying to reinvent the wheel, I thought I should take advantage
> of the vast experience that is available on this bulletin board. We wish to
> have a simple test case for MIR technique, for the benefit of new students
> in our lab. As for any good test-case scenario, we are looking for:
> i)  a protein that is commercially available,
> ii)  easily crystallizable and
> iii)  well established protocol for heavy atom derivatives should be available
>
> With many thanks, in anticipation,
>
> Hemant
>
> Hemant Yennawar Ph.D.           Phone   814 865 8383
> 6 Althouse Laboratory,                  Fax     814 863 7024
> University Park, PA 16802