Good point, my case involves the reduction of a redox center through
x-ray exposure.
I understand that all atoms may move at temperatures above 0 K but is
there enough
energy for concerted movements of residues? Obviously, freeze trapping
experiments
have been successful. I am hoping for examples where residue
conformational changes
in a frozen crystal have been documented and correlated to the radiation
exposure.
"DeLano, Warren" wrote:
How could one distinguish side chain movement from static disorder in
such a structure?
good point.
However, for oxidized redox centers that are reduced by synchrotron
radiation. Different
exposure times for multiple crystal structures that are frozen in
identical conditions
could provide such information.
-----Original Message-----
From: Paula Lario [mailto:lario@biology.ucsc.edu]
Sent: Tue 5/7/2002 8:57 AM
To: ccp4
Cc:
Subject: [ccp4bb]: Residue movements at 100K?
Hello All,
I was wondering if anyone has evidence for side chain movements
occurring
in a frozen crystal (@ 100K) or if anyone believes that it is
possible.
--
~~~~~~~~~~~~~~~~~~~~~~~~~~
Paula Lario
McGill University / UCSC
Nothing is ours, but time.
- To: "DeLano, Warren" <warren@sunesis.com>
- Subject: Re: [ccp4bb]: Residue movements at 100K?
- From: Paula Lario <lario@biology.ucsc.edu>
- Date: Tue, 07 May 2002 09:56:12 -0700
- References: <E7EE394346CE6A4B88E5B3495F8A76794EA1B7@mercury.sunesis.com>
Good point, my case involves the reduction of a redox center through x-ray exposure.
I understand that all atoms may move at temperatures above 0 K but is there enough
energy for concerted movements of residues? Obviously, freeze trapping experiments
have been successful. I am hoping for examples where residue conformational changes
in a frozen crystal have been documented and correlated to the radiation exposure."DeLano, Warren" wrote:
How could one distinguish side chain movement from static disorder in such a structure?good point.
However, for oxidized redox centers that are reduced by synchrotron radiation. Different
exposure times for multiple crystal structures that are frozen in identical conditions
could provide such information.
-----Original Message-----
From: Paula Lario [mailto:lario@biology.ucsc.edu]
Sent: Tue 5/7/2002 8:57 AM
To: ccp4
Cc:
Subject: [ccp4bb]: Residue movements at 100K?
Hello All,
I was wondering if anyone has evidence for side chain movements occurring
in a frozen crystal (@ 100K) or if anyone believes that it is possible.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~ Paula Lario McGill University / UCSC Nothing is ours, but time.-- ~~~~~~~~~~~~~~~~~~~~~~~~~~ Paula Lario McGill University / UCSC Nothing is ours, but time.