RE: [ccp4bb]: Xtals

["Sangkee Rhee" <srheesnu@snu.ac.kr>]

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Jan,

I was able to grow thicker xtals by "macro" or/and "micro" seedings from 
needle-like xtals.  Based on what you wrote, it seems like you are better to
try micro-seeding first.   You could find details on page 13-14 of "Practical
Protein Crystallography" by Duncan E. McRee.

Good luck

Sangkee
=================================
Sangkee Rhee
School of Agricultural Biotechnology
College of Agriculture & Life Sciences
Seoul National University
Seodoon-Dong, Suwon
KOREA

Phone) +82-31-290-2418
FAX) +82-31-293-8608
e-mail)  srheesnu@snu.ac.kr 

-----Original Message-----
From: owner-ccp4bb@dl.ac.uk [mailto:owner-ccp4bb@dl.ac.uk]On Behalf Of Jan Dohnalek
Sent: Tuesday, May 14, 2002 2:02 AM
To: ccp4bb
Subject: [ccp4bb]: Xtals


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Hi,
I am desparately trying to grow 'thicker' crystals of my 'needles'.
Has anyone had experience of getting this done. pH, protein
concentration,
drop size and temperature do not help. Some dependance on additives but
nothing great. 
Any useful ideas?????
(Yes, seeding also did not change the size)
Jan