[ccp4bb]: Solvent Flattened Data

[Paul Hubbard <phubbard@post.its.mcw.edu>]

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Hello all,

I am having trouble using SIGMAA on solvent flattened data, and I can't
figure out why.

I have a reasonable model which is about 70% complete, built using
solvent flattened experimental data to 3.7 Angstroms (no phase
extension). However when I use this data with the model in SIGMAA, the
map looks awful. However, when I use the un-flattened data things look
peachy. I tried swapping FP's with the native data, but no improvement.
Am I missing something here?

By the way, just out of curiosity, would it be okay to use FWT and PHFWT
(WCMB) for solvent flattening? Supposedly this is an unbiased 2Fo-Fc, so
I would suspect flattening would tidy things up. Just wondering.

Thanks

AGS

--
Paul Hubbard
Dept. of Biochemistry
Medical College of Wisconsin
Phone: 414-456 4305
Fax: 414-456 6510
URL: iris9.biochem.mcw.edu