Re: [ccp4bb]: NCS masks in DM & SOLOMON

[Kevin Cowtan <cowtan@ysbl.york.ac.uk>]

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On Tuesday 09 Jul 2002 9:52 pm, Sugoto Chakravarty wrote:
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> I have some queries regarding the masks in Dm and SOLOMON:
>
> I got the rotation and translation parameters for a multimeric protein
> using CNS/XPLOR. I wanted to get the averaged and solvent flattened
> phases and structure factors using DM & SOLOMON. That required the
> construction of appropriate masks.

No. DM will generate its own mask internally - you do not need to give one. 
You can output this mask for use in Solomon if you wish.

> 1. Can I use the same mask file for both averaging and solvent
> flattening in DM i.e.  will DM automatically adjust the masked regions
> when it switches between the averaging and solvent flattening modes? If
> not, what is the easiest way to construct a solvent flattenng mask from
> an averaging mask and vice-versa?

No.

Solvent from NCS: Run mapmask with 'expand overlap' and 'xyzlim cell'.

NCS from solvent: no general solution.

> 2. Can I use the same mask file in both DM and SOLOMON?  If not, is
> there an easy way to convert a mask from one program to the other?

I believe so, if you turn off the automatic mask determination in 'dm'.

-- 
Department of Chemistry, University of York, Heslington, York YO10 5DD