[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]

Re: [ccp4bb]: Membrane protein



***  For details on how to be removed from this list visit the  ***
***          CCP4 home page http://www.ccp4.ac.uk         ***

Narayanan Ramasubbu wrote:
> 
> ***  For details on how to be removed from this list visit the  ***
> ***          CCP4 home page http://www.ccp4.ac.uk         ***
> 

> I am working on a membrane protein which has been provided to me at 1mg/mL
> concn. I would like to find out whether or ethere are methods available to
> increase the concn. The biochemist has had no luck and suggests triton-x
> and may be beta octylglucoside. Are there other newer detergents
> available?

There are detergent kits available from several manufacturers (hampton,
boehringer, ...).
This is expensive stuff, so you want small experiments to start with :-)
Find a detergent that works for you (work at 1.2 or 1.5x the CMC).
Change detergents by dialysis.
Try to avoid triton-x, as there is no way to get rid of it afterwards. 

> Any suggestions owuld be ost welcome.
> subbu

-- 
Klaas Decanniere                        E-mail: klaas@ultr.vub.ac.be
VUB - dienst ULTR                       Phone: + 32 2 359 0267
Paardenstraat 65                        Fax:   + 32 2 359 0289
B-1640 St. Genesius Rode               
Belgie                                  Sponsored by VIB