Re: [ccp4bb]: CNS composite SA omit - summary

[<rams@poori.biochem.uiowa.edu>]

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Hi Phoebe,

APologies for the late reply.

Our experience is the same - general disillusion.  However, TOM 
in my lab with Garibs helpd used a different trick which works slightly 
better.

This uses refmac5, and does a reciporal space omit calculation.  20 
different free R-sets, each time starting from random perturbation of the 
structure.  Works well if you dont have NCS and to resolutions worse than 
about 2.2A.  If you have good high resolution data, these look like the 
refmac maps themselves (which to me is not surprising). it takes very 
little time to run compared to the composite omit map of CNS too.

Ask Tom and he will e-mail the script for you - whatever result you get we 
would like to know what happened.  

Rams.




-- 
S. Ramaswamy
Department of Biochemistry.
University of Iowa.