RE: [ccp4bb]: Re: [o-info] R-sym I/sigI etc...

["Frank Vondelft" <Frank.Vondelft@syrrx.com>]

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Ed - you're not the referee, are you?   *grin*




> -----Original Message-----
> From: Edward A. Berry [mailto:EABerry@LBL.gov]
> Sent: Sunday, August 04, 2002 11:56 AM
> Cc: ccp4bb@dl.ac.uk
> Subject: Re: [ccp4bb]: Re: [o-info] R-sym I/sigI etc...
> 
> 
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> ***          CCP4 home page http://www.ccp4.ac.uk         ***
> 
> Well ...
> Rather than omit the crystal entirely, I think I would cave in to 
> the referee's demand and use a lower resolution or a sigma cutoff 
> for the crystallization paper. This does not commit you to doing 
> the same in the structure paper, does it? Since I take it the 
> structure paper is pretty much assured now, the crystallization
> paper is not important enough to be petulant by insisting on
> using your last shell or otherwise not publishing any of it!
> You can put a note in the text that there is some data at higher
> resolution and you intend to use it in refinement, so that
> crystallographers who don't follow this BB won't think you
> old-fashioned.
> 
> Just my feelings -
> Ed
> 
> rams@poori.biochem.uiowa.edu wrote:
> > 
> > Thanks everybody, but I am not done yet....
> > 
> > The overall discussion is very useful.  WE all agree weak 
> data can be very
> > useful.... but..
> > 
> > Lots of suggestions as to how I can prove my resolution 
> with my maps and
> > refinement etc..etc.. I know I can do that.  But I dont 
> think I need to in
> > a crystallization paper.  I am just quoting what I see from 
> my crystals.
> > If I say everything about the structure, then why have a 
> crystallization
> > section in ACTA -D.  I will write my structure paper and 
> just mention in
> > passing - that my crystals were grown with PEG4000 @pH6.0 - 
> let the next
> > person (if somebody ever does) who wants to try reproducing 
> figure it out.
> > 
> > Now  prove that the data was useful - while I
> > can prove that very well (now that I have the full 
> structure refined);
> > maps improve (I can see my ligand much better). My crystal 
> is anisotropic
> > and the average I/sigI is just completely meaningless. etc...etc..
> > 
> > So the take home message - only publish crystallization 
> paper after you
> > have full structure - or publish with good numbers (even if 
> tweaked), when
> > you cannot solve the structure!!   I suppose many of the 
> powerful ACTA-D
> > editorial people I hope are seeing these messages - I 
> wonder if that was
> > the purpose of the crystallization communications....
> > 
> > Unless, I see a strong reason to fight - I am planning to 
> cut off data
> > from this crystal form. We had data in 2 crystal forms and 
> only one is
> > in question.  What I am willing to say in the bulletin 
> board, but not in
> > my CYRSTALLIZATION PAPER - is that without multiple crystal 
> averaging, we
> > would not have been able to solve the structure for now.
> > 
> > Will wait a week - cool down a bit more before I respond to 
> the referee.
> > 
> > Thanks for all the support. More flames/suggestions/support welcome.
> > 
> > Rams.
> > 
> > --
> > S. Ramaswamy
> > Department of Biochemistry.
> > University of Iowa.
> > 
> > _______________________________________________
> > o-info mailing list
> > o-info@o-info.imsb.au.dk
> > http://o-info.imsb.au.dk/mailman/listinfo/o-info
>


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