[ccp4bb]: MR with partial structure

[Laurie Betts <laurie_betts@med.unc.edu>]

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All-

We have a crystal of a complex of two proteins - one is 130 amino acids
the other is 200.  The structure of the smaller is known.  If we
correctly place the known protein by MR is it possible to see
interpretable density representing the second with phases from the
first?  If so, what coefficients should we use?  Crystals diffract to
2.2 A - I/sigma < 12 all resolution ranges but >4 to 2.2.  Space group
orthorhombic, apparently one of each protein in asymmetric unit.

Thanks

--
Laurie Betts,  Ph.D.
Facility Manager
UNC Biomolecular X-ray Facility
Dept. of Pharmacology CB#7365
UNC-Chapel Hill, 27599-7365 USA
Ph (919-843-7174)
FAX (919-843-9244)