Re: [ccp4bb]: MR with partial structure

[Paul Emsley <paule@chem.gla.ac.uk>]

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On Mon, 2002-10-07 at 15:21, Laurie Betts wrote:
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> All-
> 
> We have a crystal of a complex of two proteins; one is 130 amino acids
> one is 200.  We know the structure of the smaller one  - the larger is
> unknown.  If the known protein is correctly placed by MR should it be
> possible to see density representing the second protein with phases from
> the first?  What coefficients should we use?  Crystals diffract to 2.2 A
> at least; not terribly strong (I/sigma < 12 all resolution ranges).
> 

Laurie,

Yes.

You need buster.

Failing that you could try DM (which although routinely described by its
author as "obsolete", I believe to be of some merit at least and may be
of use to you).

(You will, I believe, need to calculate partial structure FOMs with
sigmaa before you can use DM).

HTH,

Paul.