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RE: [ccp4bb]: MR with partial structure
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Hi Laurie
Try and see :-)
If you have the exact structure of the 130aa protein, your chances are a
lot better than if it's some low homology thing. Of course. The first
question would, I think, rather be wether you *can* in fact place it
correctly; if you're unlucky, this could itself be a problem.
If you have it, and if you're lucky, Arp/Warp will build lots of the
second one. SigmaA coefficients should do the job (unless I've missed
something), but if you struggle, you should think of using Buster, which
tries to be good at this sort of thing. EDEN
(http://www.edencrystallography.com/) also tries to do this, using the
holographic method. (I have no experience with it).
> -----Original Message-----
> From: Laurie Betts [mailto:laurie_betts@med.unc.edu]
> Sent: Monday, October 07, 2002 7:28 AM
> To: ccp4 bb
> Subject: [ccp4bb]: MR with partial structure
>
>
> *** For details on how to be removed from this list visit the ***
> *** CCP4 home page http://www.ccp4.ac.uk ***
>
> All-
>
> We have a crystal of a complex of two proteins - one is 130
> amino acids
> the other is 200. The structure of the smaller is known. If we
> correctly place the known protein by MR is it possible to see
> interpretable density representing the second with phases from the
> first? If so, what coefficients should we use? Crystals diffract to
> 2.2 A - I/sigma < 12 all resolution ranges but >4 to 2.2. Space group
> orthorhombic, apparently one of each protein in asymmetric unit.
>
> Thanks
>
> --
> Laurie Betts, Ph.D.
> Facility Manager
> UNC Biomolecular X-ray Facility
> Dept. of Pharmacology CB#7365
> UNC-Chapel Hill, 27599-7365 USA
> Ph (919-843-7174)
> FAX (919-843-9244)
>
>
>
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