Re: [ccp4bb]: disordered structure

["Eleanor J. Dodson" <ccp4@ysbl.york.ac.uk>]

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OnLineHelpForm wrote:
> 
> The problem is as follows:
> I have a structure of a dimeric protein in P21.
> The structure was solved by MAD.  The maps for one half of the dimer
> were excellent.  The other half is quite disordered with large regions
> having uninterpretable density.  This seems to be due to a lack
> of crystal contacts for the disordered regions.  Just wondering if anyone
> has come across structures with this problem before?  Mainly looking
> for other literature examples.
> 
> Thanks in advance,
> Megan, m.maher@mmb.usyd.edu.au


 That certainly happens - have you tried TLS refinement? very
appropriate in such cases.

Another possibility is that the spacegroup is not quite P21 and you are
imposing extra symmetry which is true for the one molecule but not the
other - this is rare but does happen.

How well did the data merge?
 Eleanor
 
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Eleanor J.Dodson, York Structural Biology Laboratory,
                  Chemistry Department, 
                  University of York, Y01 5DD Heslington, U.K.
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