[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
[ccp4bb]: Protein/nucleic acid crystallization
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
I'm chasing a condition from a sparse matrix screen for a protein/DNA
complex. Things are going well but I'm worried about an unusually high
spermine concentration. Complex crystals grow between 10mM and 15mM
Does anyone have any experience of protein/nucleic acid crystals at this or
even higher level of spermine?
As far as I can tell the spermine does not dissociate the complex and drops
containing just the oligo do not have crystals.
Thanks in advance,
MRC Laboratory of Molecular Biology
Tel. +44 (0)1223 402444
Fax. +44 (0)1223 213556