[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
RE: [ccp4bb]: ccp4bb- Warp-Arp
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Tassos wrote:
> A small note: I always use 1000-1500 reflections for Rfree instead
> of the more 'standard' 10%. Saving on a few reflections sometimes
> for me simply does the trick.
This 10% indeed is the same unreflected number like 10mg/ml conc
for crystallization. Meth enz 277 B pp 382 essentially states
in more cryptic words that 'as many reflections as necessary to make
free R statistically meaningful' i.e., the error in Rfree reasonable.
That std error (p384) appears to be Rfree/sqrt(n), with n the number of
test
reflections, and not a percentage.
Handy ref in case one needs to respond to a review comment.
BR