[ccp4bb]: PEG/isopropanol crystals

[<gborgstahl@unmc.edu>]

Hello ccp4-ers,  
Sorry for yet another non-ccp4 related question.

We have beautiful crystals grown from 9% PEG4000 and 6% isopropanol.
I anticipate that they may be difficult to freeze in the loop due to the evaporation of isopropanol.

Does anyone have any suggestions for cryocooling these crystals.
I've thought to try the various oils.  But perhaps we need to substitute the isopropanol 
with another organic solvent by soaking.  

Has anyone had any success cryocooling isopropanol grown crystals?

Thank you in advance, Gloria

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Gloria Borgstahl                                            
Eppley Institute for Cancer Research
987696 Nebraska Medical Center   
10732A Lied Transplant Center               
Omaha, NE 68198-7696

Office (402) 559-8578
FAX (402) 559-8577

Associate Professor                
Interests:  Cancer, Biochemistry, DNA Metabolism, 
Protein Crystallography, Super-fine phi slicing, Synchrotron 
Radiation, Crystal Mosaicity, and X-ray Topography
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