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RE: [ccp4bb]: PEG/isopropanol crystals
Hi
Gloria,
See
whether you can increase the PEG4K to around 24-26% and it should freeze.
24-26%PEK4K with HEPES and upto 0.2 salts work freezes well and, I think,
you may be able to go to a lower PEG concentration with your isopropanol
condition. Since PEG4K is already in the medium, crystals may tolerate
higher concentration.
good
luck,
Mathews
Hello ccp4-ers,
Sorry for yet another non-ccp4
related question.
We have
beautiful crystals grown from 9% PEG4000 and 6% isopropanol.
I anticipate that they may be difficult to freeze in
the loop due to the evaporation of isopropanol.
Does anyone have any suggestions for cryocooling these
crystals.
I've thought to try the
various oils. But perhaps we need to substitute the isopropanol
with another organic solvent by
soaking.
Has anyone had any
success cryocooling isopropanol grown crystals?
Thank you in advance, Gloria
******************************************************************************
Gloria
Borgstahl
Eppley Institute for Cancer Research
987696 Nebraska
Medical Center
10732A Lied Transplant Center
Omaha, NE 68198-7696
Office (402)
559-8578
FAX (402) 559-8577
Associate Professor
Interests: Cancer, Biochemistry,
DNA Metabolism,
Protein Crystallography, Super-fine phi slicing,
Synchrotron
Radiation, Crystal Mosaicity, and X-ray
Topography
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