[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
Re: [ccp4bb]: R32 pseudotranslational symmetry in crystal
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Carredano, E., Kauppi, B., Choudhury, D., and Ramaswamy, S. (2000)
Psuedosymmetry characterisation in the rhombohedral crystal form of
naphthalene dioxygenase. Acta Crystallographica D56: 313-321.
Will tell you, how to test and correct....
On Tue, 25 Feb 2003, N.Manoj wrote:
> *** For details on how to be removed from this list visit the ***
> *** CCP4 home page http://www.ccp4.ac.uk ***
> Dear all,
> We have a problem in refining a structure in space group R32.
> The data processed to 2.9A with overall Rmerge of 5%, 98% completeness
> and redundancy of 5. AMoRe gave clear solutions for the two expected
> molecules in the asymmetric unit. The packing looked good, but the
> structure could not be refined below 45% R-factor. Electron density for
> one monomer is good while it is very poor for the other monomer.
> The two molecules are identical (same rotation function values) in
> orientation and are translated by almost 1/2 along the Z axis. I would
> like to know if there is some way of going about refining this
> I remember some discussion about refinement of structures with pseudo
> translational symmetry in the ccp4 BB some time back.
> Thanks in advance
Department of Biochemistry.
University of Iowa.