Re: [ccp4bb]: Link-statement

[Goetz <dgoetz@fhcrc.org>]

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Dear Florian,
	I use the following link statement in my refmac refinement runs:
LINK        OG   SER A 199        1.400   CF   MAI B   3                
SER-MAI

(My e-mail program breaks this up into two lines, but it should just be 
one line)

This creates a 1.4A link between Serine Chain A residue 199 Atom OG and 
MAI Chain B atom CF.

Then in my command file for refmac I make sure I have the following line:
make link Y


On Thu, 27 Feb 2003, Florian Schmitzberger wrote:

> Dear all,
> 
> I have a problem when refining a protein-structure in refmac with ccp4-4.2.1. I have a covalently attached peptide-moiety bound to my protein. I was trying to connect the two residues, which are connected, by putting "MAKE LINK" or  "LINK" in the header of my pdb and specifying the two residues. But after the refinement it removed the "MAKE LINK" or "LINK"-statement in the header and did not connect the two parts. I am suspecting I am not using the right keyword or doing something else stupidly wrong.  I have also tried to specify the atoms in the two residues which I want to connect, but still have the same problem. 
> 
> I would appreciate any advice what I am doing wrong and what I have to do to succeed in linking the two parts.
> 
> Thanks very much,
> 
> Florian
> ----------------------------------
> Florian Schmitzberger
> Department of Biochemistry
> University of Cambridge
> 80 Tennis Court Road
> CB2 1GA Cambridge
> Tel: +44 1223 766029