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Dear all,
I have a problem when refining a
protein-structure in refmac with ccp4-4.2.1. I have a covalently attached
peptide-moiety bound to my protein. I was trying to connect the
two residues, which are connected, by putting "MAKE LINK" or
"LINK" in the header of my pdb and specifying the two residues. But
after the refinement it removed the "MAKE LINK" or "LINK"-statement in the
header and did not connect the two parts. I am suspecting I am not using the
right keyword or doing something else stupidly wrong. I have also tried
to specify the atoms in the two residues which I want to connect, but
still have the same problem.
I would appreciate any advice what I am
doing wrong and what I have to do to succeed in linking the two
parts.
Thanks very much,
Florian
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Florian Schmitzberger Department of Biochemistry University of Cambridge 80 Tennis Court Road CB2 1GA Cambridge Tel: +44 1223 766029 |