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[ccp4bb]: Crystals Crack in Cryo !!
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I am working with a mutant of my WT molecule. It crystallizes in similar
conditions as WT. However, when my crystals are soaked in the same cryo as
that of WT, the crystals develop cracks. These cracked crystals diffract to
about 3.5 Ang. Although seemingly fine at indexing and scaling stage), MR
attempts with such data have failed.
The normal cryosoak involves stepwise soaking for a few mins. in increasing
concentrations of MPD solution (final 24% MPD). I have tried varying final
concentrations and times of soak in MPD at each step. In all these cases, the
crystals develop cracks in a few hours.
I also screened for various alternate cryoprotectants; in any other cryo, my
crytals completely lose diffraction - they are essential "dead" for
Does anyone have any suggestions/clues as to how I should handle this issue?
Thanks very much,
Dept. of Biochemistry & Molecular Biology
Colorado State University
Fort Collins, CO 80523 USA