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Dear Tristan
- one possibility is that the 'lost' domains do not conform to all
the symmetry operations of your spacegroup - so you made a 'mistake' (so
far as those domains were concerned) when you merged all the reflections.
Did you have a 'difficult' choice between a lower and higher symmetry
spacegroup during data reduction? The down side of exploring this is you
will need a model for an 'expanded' asu with multiple copies of the 'good'
domains which maybe dominate the data. If you decide to proceed this way
you must make sure your Rfree set contains all 'pseudo'-symmetry related
measurements otherwise the refinement program gets a 'sneak preview' of
the Rfree dataset from unflagged data that are highly correlated with it.
And you will have to set very strict NCS on the 'good' domains. So if your
maps are not noticeably better calculated from the lower symmetry group
then it is not worth following up.
- I remember Prof. Lawrence Pearl describing this happening for a
DNA:protein complex where too high a symmetry was imposed that only one
component really satisfied.
- I would be interested to hear if anyone has had this happen with
multidomain proteins.
- good luck
Martyn
On Thu, 20 Mar 2003, Tristan Fiedler wrote:
Date: Thu, 20 Mar 2003 14:56:12 -0500
From: Tristan Fiedler <tfiedler@newssun.med.miami.edu>
Cc: ccp4bb@dl.ac.uk
Subject: [ccp4bb]: Missing Domain Search
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Dear CCP4 a/o CNS users,
We are working with a MAD dataset with phase information extending to
2.0 Angstrom resolution which has allowed unambiguous modelling of 250
out of 350 residues. Current R/Rfree are high 20's/low 30's without
modeling any waters.
The problem is that the N-terminal domain of ~100 residues can not be
located visually in the density using O. Homologs of the N-terminal
domain exist in the PDB. Testing CNS:rigid.inp using a homolog was
unsuccessful.
Any suggestions as to how to locate the missing N-terminal domain would
be appreciated. We realize it may (most likely is) disordered and no
density may exist in the maps.
Thank you in advance,
Tristan Fiedler
Mark Del Campo
--
Tristan J. Fiedler, Ph.D.
Malhotra Laboratory
Department of Biochemistry & Molecular Biology
University of Miami Medical School
t.fiedler@umiami.edu
tfiedler@newssun.med.miami.edu
Martyn F. Symmons
Research Associate
Crystallography and Biocomputing Group
Department of Biochemistry
University of Cambridge
Phone: 01223 766020
-----------------------
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