*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Dear All, I have a so effeminate protein now. It would precipitate when dialyzing. I spent all my affection and did all I could then get a solution at low concentration. After that, when I began to concentrate it, my dear protein precipitate again. What can I do? Regards, Li Sheng