Dear all, I got hexagonal crystals of a protein. My previous data show the space is P6122. Recenly I put one of my best crystals for data collection. The frame looks good (I have attached it). And I use DENZO program to index the data. All previous step go on smoothly, until I scale the data. The scale result is very bad ( Part is attached). The linear R factor is about 0.516 and square R factor is 0.922 and the chi**2 is obviously wrong. But I notice that the chi**2 of each frame during process is near 1 which is OK. Could anyone help me to figure out the possible cause? Thanks a lot!
Ms yuan Ping Macromolecular x-ray Crystallography Group Institute of Molecular Agrobiology 1 Research Link National University of Singapore Singapore 117604 Tel: 8727407(lab) 8727409(0ffice) 7787754(Home) Fax: 8727007