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[ccp4bb]: membrane prot x-tal problem

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dear users,

i have recently beein trying to xtalize a 50kda membrane protein
and have had limited success. using peg 4000, and ammonium sulfate
i can get a few nice crystals. adding 1,6-hexanediol and some
alcohol improves this. however, the crystals dissolve almost as 
quickly as they appear, after one or two days. i've tried detergent
screens and additive screens but without success. does anyone know
how i might stabilize these xtals? any advise on membrane proteins
specifically would be a great help. 

stephen kelly
dept of microbiology
univeristy of alabama at birmingham

Songlin Li, Ph.D.
Dept. Microbiology
University of Alabama at Birmingham
AL35294, USA
Email: songlin@credit.cmc.usb.edu