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Re: [ccp4bb]: Co-crystallisation with hydrophobic ligands
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On Mon, 2 Oct 2000, Leo James wrote:
> Im wondering if anyone has any clever strategies for co-crystallising
> complexes with very hydrophobic ligands. The ligands I am using are
> insoluble at concentrations upwards of 100-200ÁM in buffer and are
> soluble in DMSO only up to 50mM.
one obvious approach would be to make the complex in dilute solution, and
then concentrate the complex, rather than adding ligand to protein thats
"I am an omnivorous reader with a strangely retentive memory for trifles"
-- Sherlock Holmes
David J. Schuller
modern man in a post-modern world
University of California-Irvine