Re: [ccp4bb]: Co-crystallisation with hydrophobic ligands

["David J. Schuller" <schuller@uci.edu>]

***  For details on how to be removed from this list visit the  ***
***    CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html    ***

On Mon, 2 Oct 2000, Leo James wrote:

> Im wondering if anyone has any clever strategies for co-crystallising
> complexes with very hydrophobic ligands.  The ligands I am using are
> insoluble at concentrations upwards of 100-200µM in buffer and are
> soluble in DMSO only up to 50mM.

one obvious approach would be to make the complex in dilute solution, and
then concentrate the complex, rather than adding ligand to protein thats
alraady concentrated.

=======================================================================
"I am an omnivorous reader with a strangely retentive memory for trifles"
                                                      -- Sherlock Holmes
=======================================================================
                        David J. Schuller
                        modern man in a post-modern world
                        University of California-Irvine
                        schuller@uci.edu