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Re: [ccp4bb]: low-temp data collection
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Our laboratory has used oil, in place of a cryprotectant, for cubic lipid
phase bacteriorhodopsin crystals successfully...
JP@UCI
>
> Hopefully it is not a membrane protein you need to freeze, oil drop would
> not work in this case for obvious reasons.
> Good luck,
> Salam
>
>
> >
> >Using an oil drop in place of cryoprotectant (I think this was suggested
> >on this list some time ago) should also protect the crystal from air.
> >You can walk around with the blob on a cryoloop at leasure before
> >cooling with no evaporation. Be careful to wick away any excess solvent
> >still stuck to the crystal faces inside the oil drop. The drop should form a
> >perfectly clear glass ... no white patches. We've just seen spectacular
> reduction in
> >mosaic spread on the couple of cases here at MacCHESS the other day.
> >
> >
> >Richard Gillilan
> >MacCHESS
> >Cornell UNiversity
> >
> >
> >
>
>
>
> --------
> Salam Al-Karadaghi,
> Molecular Biophysics, Lund University
> PO Box 124, 221 00 Lund,
> Sweden
>
> Tel. Int+46 (0)462224512
> Fax. Int+46 (0)462224692
> Web: http://www.mbfys.lu.se
> --------
>