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Re: [ccp4bb]: low-temp data collection
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Hopefully it is not a membrane protein you need to freeze, oil drop would
not work in this case for obvious reasons.
Good luck,
Salam
>
>Using an oil drop in place of cryoprotectant (I think this was suggested
>on this list some time ago) should also protect the crystal from air.
>You can walk around with the blob on a cryoloop at leasure before
>cooling with no evaporation. Be careful to wick away any excess solvent
>still stuck to the crystal faces inside the oil drop. The drop should form a
>perfectly clear glass ... no white patches. We've just seen spectacular
reduction in
>mosaic spread on the couple of cases here at MacCHESS the other day.
>
>
>Richard Gillilan
>MacCHESS
>Cornell UNiversity
>
>
>
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Salam Al-Karadaghi,
Molecular Biophysics, Lund University
PO Box 124, 221 00 Lund,
Sweden
Tel. Int+46 (0)462224512
Fax. Int+46 (0)462224692
Web: http://www.mbfys.lu.se
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