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Re: [ccp4bb]: low-temp data collection
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1. In case you didn't think of mounting in capillaries (otherwise
skip to 2.):
Mount from dithionite solution into capillaries using glove box and
intert athmosphere e.g. nitrogen, no need for stabilization buffer.
Freeze capillaries in liquid propane. Works very well.
2. If you work quickly fishing Mb-deoxy crystal from dithionite and freeze it
air does not make a difference at all (extensive work with deoxy-Mb by
one colleague from the lab. (V. Srajer) using a microspectrophotometer).
3. only an idea: freeze Mb-deoxy in loop in liquid nitrogen in glove box. Transfer
loop in vial filled with liquid nitrogen to cryostream.
Dr. Marius Schmidt
The University of Chicago
Biochemistry and Molecular Biology
Cummings Life Science Center
920 East 58th Street
5709 South Maryland Avenue
Tel (office) : +1-773-702-1801
Fax (office) : +1-773-702-0439
Tel (home) : +1-773-288-0872
On Fri, 5 Jan 2001, Martin K. Safo wrote:
> *** For details on how to be removed from this list visit the ***
> *** CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html ***
> Sorry to post this here, but could somone help me on this. How do you
> collect a low-temperature dataset with a deoxyhemoglobin crystal without
> exposing the crystal to atmospheric air. Thank you.
> Martin Safo, Ph.D