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Re: [ccp4bb]: low-temp data collection

To: "Martin K. Safo" <msafo@hsc.vcu.edu>
Subject: Re: [ccp4bb]: low-temp data collection
From: "J. Tame" <jtame@npn.jst.go.jp>
Date: Sat, 06 Jan 2001 07:24:13 +0900
CC: "ccp4bb@dl.ac.uk" <ccp4bb@dl.ac.uk>
References: <3A55F3BD.B48E09BA@hsc.vcu.edu>
Sender: owner-ccp4bb@dl.ac.uk

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Look at 
"The structures of deoxy human haemoglobin and the mutant
Hb Tyrosine a42->His at 120K"
Tame and Vallone, Acta Cryst D56, 805-811

It is possible to protect the crystals from oxygen using dithionite,
at least long enough to cryo-cool them.

>>Sorry to post this here, but could somone help me on this.  How do you
collect a low-temperature dataset with a deoxyhemoglobin crystal without
exposing the crystal to atmospheric air.  Thank you.

-- 
Jeremy Tame
Protonic Nanomachine Project 
ERATO, 3-4 Hikaridai, Seika, Kyoto 619-0237 Japan
Phone: 81-774-98-2543  Fax: 81-774-98-2575  E-mail: jtame@npn.jst.go.jp

References:
- [ccp4bb]: low-temp data collection
  - From: "Martin K. Safo" <msafo@hsc.vcu.edu>

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