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[ccp4bb]: disulfide isomerase and refolding!
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Once again a non-ccp4 related (?) pre-crystallography question.
Have people succeded in using PDI to successfuly refold proteins
from e.coli overexpressed mixed disulfide scenario.
I have a protein that has 7 disulfides and it is expressed in E.Coli.
The disulfides are a mixture of all kinds as seen from peptide mapping. I
want to unfold them and refold them with PDI (I seem to have heard that
this works). How can one do this....
S.Ramaswamy Phone: 319 335 7917
Department of Biochemistry, Fax : 319 335 9570
University of Iowa, IA 52242.