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Re: [ccp4bb]: disulfide isomerase and refolding!

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>Have people succeded in using PDI to successfuly refold proteins
>from e.coli overexpressed mixed disulfide scenario.

Have a look at:

Martens E, Alloza I, Scott CJ, Billiau A, Vandenbroeck K
Eur J Biochem 2000 Nov;267(22):6679-83
Protein disulfide isomerase-mediated cell-free assembly of 
recombinant interleukin-12 p40 homodimers.

Mayer M, Kies U, Kammermeier R, Buchner J
J Biol Chem 2000 Sep 22;275(38):29421-5
BiP and PDI cooperate in the oxidative folding of antibodies in vitro.

van den Berg B, Chung EW, Robinson CV, Mateo PL, Dobson CM
EMBO J 1999 Sep 1;18(17):4794-803
The oxidative refolding of hen lysozyme and its catalysis by protein 
disulfide isomerase.

Hope this helps,

  Luca Jovine, Ph.D.
  Department of Biochemistry and Molecular Biology
  Mount Sinai School of Medicine
  Annenberg Building, Room 25-18
  One Gustave L. Levy Place, New York, NY 10029-6574, U.S.A.
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  E-Mail: jovinl02@doc.mssm.edu  W3: http://www.mssm.edu/students/jovinl02