Re: [ccp4bb]: disulfide isomerase and refolding!

[Luca Jovine <jovinl02@doc.mssm.edu>]

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>Have people succeded in using PDI to successfuly refold proteins
>from e.coli overexpressed mixed disulfide scenario.

Have a look at:

Martens E, Alloza I, Scott CJ, Billiau A, Vandenbroeck K
Eur J Biochem 2000 Nov;267(22):6679-83
Protein disulfide isomerase-mediated cell-free assembly of 
recombinant interleukin-12 p40 homodimers.

Mayer M, Kies U, Kammermeier R, Buchner J
J Biol Chem 2000 Sep 22;275(38):29421-5
BiP and PDI cooperate in the oxidative folding of antibodies in vitro.

van den Berg B, Chung EW, Robinson CV, Mateo PL, Dobson CM
EMBO J 1999 Sep 1;18(17):4794-803
The oxidative refolding of hen lysozyme and its catalysis by protein 
disulfide isomerase.

Hope this helps,

Luca
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