Re: [ccp4bb]: what's the best way to combine the phases from varioussources?

[<rams@poori.biochem.uiowa.edu>]

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Hi Qian,

The simple easy method that works is just to write out the H-L
coefficients for each individual refinement.  Then simply add them up
using SFTOOLS. The HL coefficients (phases) are very robust and
non-isomorphism bothers them very little.

Rams.


On Tue, 26 Jun 2001, Qian Xu wrote:

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> 
> Dear all,
> 
> I have many datasets for a protein from various sources, including
> MAD, SIRAS and MIR from different derivatives. Some of them are
> not isomorphous. I am just wondering whether there is any way by which I
> could refine and phase all of these derivatives in one single run of
> MLPHARE? (One problem is that I can't define different "natives" for
> different datasets, which I believe is necessary). If I can't do that,
> what's the best way to combine all of those phases from various sources? I
> know sigmaa can combine two sets of MIR phases. Is there any other program
> which can do this? and anything I ought to know for optimizing phase
> combination?   
> 
> Thanks in advance.
> 
> regards,
> Qian Xu
> Dept. of Physiology and Biophysics
> Boston Univ. School of Medicine
> Tel: (617)638-4083
> Fax: (617)638-4041
> E-mail: qianxu@bu.edu
>