[ccp4bb]: planarity restraint in refmac5

[Huiying Li <hli@bragg.bio.uci.edu>]

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After running 10 cycles of refinement with REFMAC5 through CCP4i GUI, I
have noticed the planarity of all the aromatic side chains (Phe, Tyr,
Trp) was distorted. Is this normal as a 0.02A deviation from the
planarity is allowed in the library file, mon_lib_prot.cif, or had I done
something wrong?

The related settings I used:
Setup Restraints: "Do not check anything"
Scaling Fobs and Fc: Bulk solvent scaling and Apply anisotropic scaling
Other parameters: Damp shifts Pdamp 0.5 Bdamp 0.5 
Geometric parameters: all the restraints was turned on except NCS

Thanks for any help.

Huiying Li
--------------------------------------------------
Huiying Li, Ph. D
Department of Molecular Biology and Biochemistry
University of California at Irvine
Irvine, CA 92697, USA
Tel: 949-824-4322(or -1797);  Fax: 949-824-3280
email: hli@bragg.bio.uci.edu
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